Gibberellin Signaling Controls Cell Proliferation Rate in Arabidopsis

By visualizing the cell cycle marker cyclinB1::b-glucuronidase in GA-signaling mutants, we show that GA modulate cell cycle activity in the root meristem via a DELLA proteins -dependent mechanism

Patrick Achard; Andi Gusti; Soizic Cheminant; Malek Alioua; Stijn Dhondt; Frederik Coppens; Gerrit T.S. Beemster; Pascal Genschik

2009

Scholarcy highlights

  • We found that at 10 days after sowing, sly1-10 plants exhibited a reduction of 41% of the leaf blade area compared to wild-type plants, whereas in the quadruple-DELLA proteins mutant, we observed an increase of 35%
  • Supporting this observation, the cell division rate representing the total number of cells produced per unit of time and per meristematic cell was, respectively, lower in sly1-10 leaves and higher in quadruple-DELLA mutant leaves than in wild-type leaves at earliest time points
  • The prolonged cell division activity observed in sly1-10 and shortened activity in quadruple-DELLA mutant leaves could be explained by a compensatory mechanism for the respective mutant’s deficit or excess cell production
  • We found that accumulation of DELLAs repressed root meristem size
  • Similar results were obtained on dissected root tips material, suggesting that the DELLA-dependent control of Kip-related protein 2 and SIM genes expression occurs in root meristematic cells
  • Observation of Stem Cell Niche Reporter Lines quiescent center-46, pSCR:SCR-GFP, and pSHR:SHR-GFP lines were grown on MS-agar plates for 5 days in a growth chamber, transferred to MS liquid medium for 2 days before to be treated by 2 mM GA or 1 mM PAC for 24 hr

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