Yttrium vanadates based ratiometric fluorescence probe for alkaline phosphatase activity sensing

Alkaline phosphatase is an important biomarker for diagnosis, and the abnormal level of serum ALP is closely related to a variety of diseases

Wei Xiao; Fang Liu; Gen-Ping Yan; Wei-Guo Shi; Kang-liang Peng; Xue-qing Yang; Xiao-jing Li; Hou-chuan Yu; Zhi-ying Shi; Hui-Hui Zeng

2019

Scholarcy highlights

  • Alkaline phosphatase is an important biomarker for diagnosis, and the abnormal level of serum ALP is closely related to a variety of diseases
  • A ratiometric fluorescence probe based on hybrid nanoparticles4: Dy3+ nanoparticle is introduced for alkaline phosphatase activity determination
  • The4: Dy3+ probe is constructed by the carbon dots and YVO4: Dy3+ through a simple mixing method, in which the blue emission of CDs at 405 nm acts as the calibrated signal, the green emission of YVO4: Dy3+ at 574 nm decreased with the increasing targets ALP, and used as the output signal
  • In the presence of analyts ALP, ALP catalyzes the hydrolysis of PPi, causing the release of Cu2+, resulting in the Dy3+ ions emission quenched, while the CDs emission at 405 nm retained unchanged, based on this, we designed the off-on-off ratiometric fluorescence platform for ALP sensing
  • The experiment result shows that the ratio of F574/F405 is linear to the concentration of ALP in arange of 0.05∼3000 U/L with a detection limit of 0.04 U/L, which is comparable or better than those reported fluorescence probe, especially the calibrated signal introduction of CDs can eliminate the background interference, improve the accuracy of proposed probe greatly
  • The discrimination of Alkaline phosphatase enzyme inhibitor with the IC50 of 26 μM, and ALP concentration in real human serum sample has demonstrated the applicability of4: Dy3+ fluorescence sensor well

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