Improving sensitivity and specificity of capturing and detecting targeted cancer cells with anti-biofouling polymer coated magnetic iron oxide nanoparticles

We report the development of ligand-functionalized anti-biofouling polyethylene glycol-block-allyl glycidyl ether polymer coated magnetic iron oxide nanoparticles for improved targeting and capturing small numbers of tumor cells in the presence of a large excess of non-target cells

Run Lin

2016

Scholarcy highlights

  • Metastasis accounts for 90% of cancer-related deaths and is a central focus in cancer management. Tumor cells can migrate from the primary site through the circulatory systems, and spreading to other organs
  • To further test whether anti-biofouling iron oxide nanoparticles can maintain high efficiency and specificity in separating targeted rare cells in more a sparse, clinically relevant blood sample, fluorescein isothiocyanate-TfIONP was incubated with 100 D556 medulloblastoma cells spiked into 1 mL of whole porcine blood at 37 °C in a 2-mL Eppendorf centrifuge tube with an iron concentration of 0.2 mg/mL
  • The PEG-b-AGE amphiphilic polymer allows for transferring hydrophobic oleic acid coated IONPs from organic solvent to water, and stabilizes the coated IONPs resulting in the mono-dispersion of nanoparticles with a narrow distribution of hydrodynamic sizes for further applications in aqueous solutions
  • Tf was conjugated with magnetic IONPs as the ligand for targeting transferrin receptor over-expressed cells
  • Using PEG-b-AGE polymer coating, anti-biofouling Tf conjugated PEG-b-AGE coated IONPs reported in this work exhibited much less protein adsorption on the surface compared with Tf-SHP
  • The excellent performance of this system is likely due to the significantly reduced protein corona formation on the surface of nanoparticles as a result of the antibiofouling polymer coating and off-target capture of “background” non-specific cells
  • By applying the anti-biofouling PEG-b-AGE polymer coating to IONPs, improved sensitivity and specificity were achieved in biomarker-directed cell capture and separation with magnetic iron oxide nanoparticles
  • With anti-biofouling properties, developed iron oxide nanoparticles may offer ameliorated capability for capturing targeted cells, such as circulating cancer cells, in biofluid samples with high sensitivity and specificity

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