Discrimination of Moutan Cortex from different sources and geographical origins for quality evaluation using microsatellite markers coupled with chemical analysis

The aim of this study is to develop specific SSRs by transcriptome analysis to distinguish Coetex Moutan samples from different sources and quantify the contents of paeonol and paeoniflorin to evaluate its chemical qualities

Yingchun Zhang

2020

Scholarcy highlights

  • The root bark of tree peony, called Coetex Moutan, is a traditional Chinese medicine
  • The identified SSR markers could be effectively applied to reflect the genetic relationship of Cortex Moutan
  • The developed method based on SSR and chemical profiles could distinguish Cortex Moutan from different sources and areas
  • CM from different cultivars or grown in various geographic areas are significantly different in quality and chemical properties
  • The aim of this study is to develop specific SSRs by transcriptome analysis to distinguish CM samples from different sources and quantify the contents of paeonol and paeoniflorin to evaluate its chemical qualities
  • 6654 SSR markers were developed in the coding regions by transcriptome. 10 pairs of SSRs were found to be consistently polymorphic when applied in all fifty individuals collected from three typical producing areas
  • The chemical profiles combined with the genetic fingerprint are proved to be effective and could precisely authenticate species and discriminate quality of Coetex Moutan

Need more features? Save interactive summary cards to your Scholarcy Library.