Overexpression of miR-142-3p improves mitochondrial function in cardiac hypertrophy

We investigated whether overexpression of miR-14 2-3 p can inhibit the expression of gene SH2B1, ameliorate cardiac mitochondrial dysfunction and cardiac hypertrophy

Bei-lei Liu; Mian Cheng; Shan Hu; Shun Wang; Le Wang; Xin Tu; Cong-xin Huang; Hong Jiang; Gang Wu

2018

Scholarcy highlights

  • Background and PurposeOur previous studies have shown that Src homology 2 B adaptor protein 1 plays an important role in cardiac hypertrophy, but the specific mechanism remains to be studied
  • Afterwards, using angiotensinII, miRNA mimic- and miRNA mimic nc- treated cardiomyocytes for a given time. α-actin staining found that the myocardial cells became larger, The expression of atrial natriuretic peptide, brain natriuretic peptide, β-Myosin Heavy Chain messenger RNA increased by polymerase chain reaction, which proved that AngII-induced cardiac hypertrophy was successfully constructed
  • The mitochondrial density was measured using mitochondrial Mito-Red staining by Confocal microscope, the mitochondrial membrane potential was evaluated using flow cytometry, Mitochondrial respiration oxygen consumption rate was measured by a Seahorse Extracellular Flux Analyzer XF96, and the expression levels of miR-14 2-3 p, ANP, BNP, β-MHC mRNA, SH2B1 in the cardiomyocytes of different groups were measured by RT-PCR and Western blotting
  • When the pressure overload myocardial hypertrophy model was constructed for four weeks, echocardiography revealed that the heart volume, Left ventricular end diastolic diameter(LVIDd), Left ventricular end systolic diameter, Left ventricular posterior wall thickness, Systolic left ventricular posterior wall, Left ventricle Mass increased, Ejection fraction % decreased of AB group increased, but transfected with miR-14 2-3 p agomir of AB, these increase was not significant, EF% reduction was not obvious
  • PCR analysis showed that the expression of ANP, BNP,β-MHC mRNA was significantly increased in AB group, but the miR-14 2-3 p agomir treatment of AB group was not significantly increased
  • During AngII-induced cardiomyocyte hypertrophy, ANP, BNP,β-MHC mRNA expression was increased, while these factors was not significantly increased in miR-14 2-3 p mimic treatment group; mitochondrial membrane potential, mitochondrial density and OCR was significantly decreased in AngII treated group, and these were not significantly reduced in miR-14 2-3 p mimic treatment group; Conclusions miR-14 2-3 p mitigate cardiac hypertrophy by directly inhibit the expression of gene SH2B1, and can protect mitochondrial function in cardiac hypertrophy of vitro and vivo
  • During AngII-induced cardiomyocyte hypertrophy, atrial natriuretic peptide, brain natriuretic peptide,β-Myosin Heavy Chain messenger RNA expression was increased, while these factors was not significantly increased in miR-14 2-3 p mimic treatment group; mitochondrial membrane potential, mitochondrial density and oxygen consumption rate was significantly decreased in AngII treated group, and these were not significantly reduced in miR-14 2-3 p mimic treatment group; Conclusions miR-14 2-3 p mitigate cardiac hypertrophy by directly inhibit the expression of gene SH2B1, and can protect mitochondrial function in cardiac hypertrophy of vitro and vivo

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