Cell specific cytotoxicity and uptake of graphene nanoribbons

We report the cytotoxicity screening of oxidized graphene nanoribbons watersolubilized with PEG-DSPE, using six different assays, in four representative cell lines; Henrietta Lacks cells derived from cervical cancer tissue, National Institute of Health 3T3 mouse fibroblast cells, Sloan Kettering breast cancer cells and Michigan cancer foundation-7 breast cancer cells

Sayan Mullick Chowdhury; Gaurav Lalwani; Kevin Zhang; Jeong Y. Yang; Kayla Neville; Balaji Sitharaman

2012

Scholarcy highlights

  • Graphene, a two-dimensional carbon nanostructure, has attracted a great deal of attention due to its unique nanoscopic properties, and has shown potential for various material, and biomedical science applications
  • The interlayer spacing of graphene sheets measured from high resolution transmission electron microscopy micrograph is ~ 0.34 nm, which is in agreement with the values reported for pristine graphene
  • The alamar blue, neutral red, trypan blue, and lactate dehydrogenase assay assay results of Henrietta Lacks cells cells taken together indicate that the oxidized graphene nanoribbons-PEG-DSPE formulations affect the cell viability at all concentrations and time points
  • O-GNR coated with PEG-DSPE show increased solubility, and stability in biological media
  • TEM images of cell histology specimens show high uptake of the O-GNR-PEG-DSPE formulations into HeLa cells compared to other cells, which show very little or no uptake
  • The results of this study suggest that O-GNRs synthesized from CNTs have a cell-specific cytotoxic effect and significantly different cytotoxicity profile compared to graphene nanoparticles prepared by the modified Hummer’s method or its variations
  • The results of this study suggest that oxidized graphene nanoribbons synthesized from CNTs have a cell-specific cytotoxic effect and significantly different cytotoxicity profile compared to graphene nanoparticles prepared by the modified Hummer’s method or its variations

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