Chronic label-free volumetric photoacoustic microscopy of melanoma cells in three-dimensional porous Scaffolds

We demonstrate photoacoustic microscopy of the spatial distribution and temporal proliferation of cells inside three-dimensional porous scaffolds with thicknesses over 1 mm

Yu Zhang


Scholarcy highlights

  • Tissue engineering is a rapidly developing, interdisciplinary field involving life sciences, materials science, and engineering
  • We have successfully demonstrated the capability of photoacoustic microscopy to non-invasively image a whole cell/scaffold construct more than 1 mm thick, resolving spatial distribution of cells in a 3D manner
  • We have demonstrated that PAM could be a powerful tool for investigating cell distributions in 3D scaffolds in a non-invasive manner
  • Melanoma cells were used as a model cell line due to their intrinsic dark pigment
  • It was confirmed that the continuous quantitative data obtained from PAM matched well with those measured using the biochemical MTT cell viability analysis
  • After continuous monitor for 2 weeks, the melanoma cells still had a high viability of over 70%
  • We believe that PAM will become a useful technique as an imaging modality for tissue engineering applications, especially when thick cell/scaffold constructs are involved, and this modality can be extended to image other cell types labeled with contrast agents such as organic dyes

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