Addressing the problem of cationic lipid-mediated toxicity: The magnetoliposome model

We found that modifying the type and amount of cationic lipid influenced both uptake efficiency and cytotoxicity of the MLs

Stefaan J.H. Soenen

2009

Scholarcy highlights

  • Phospholipids are widely used in many biomedical applications dealing with the cellular delivery of drugs, RNA or DNA vectors,,, or as coating molecules for various substances such as quantum dots or iron oxide cores,
  • For transfection and cell labelling purposes, synthetic cationic lipids are often added, but in most studies, little attention has been paid to their cytotoxic effects
  • The former seem to be caused by the generation of reactive oxygen species leading to a Ca2+ influx at high ROS levels. The latter are due to a destabilisation of the cell plasma membrane upon transfer of the cationic lipid from the ML bilayer into the plasma membrane
  • The issue of cationic lipid-mediated toxicity is addressed, using classical magnetoliposomes, i.e. 14.0 ± 0.3 nm iron oxide cores individually enwrapped by a phospholipid bilayer
  • Classical MLs and their iron oxide-free vesicular counterparts were used as a model system
  • It was shown that cationic lipid-mediated toxicity depends on the nature of the lipid, the size and physico-chemical characteristics of the liposome formulations as all these factors influence the potential escape of the cationic lipid from the nanoparticle and its subsequent translocation to the cell plasma membrane
  • The use of distearoyl-3-trimethylammonium propane instead of dioleoyl-3-trimethylammonium propane or the use of SM

Need more features? Save interactive summary cards to your Scholarcy Library.