N-butyryl-homoserine lactone, a bacterial quorum-sensing signaling molecule, induces intracellular calcium elevation in Arabidopsis root cells

Our observations suggest that C4-HSL may act as an elicitor from bacteria to plants and that Ca2+ signaling participates in the ability of plant cells to sense the bacterial quorum sensing signals

Shuishan Song; Zhenhua Jia; Jinze Xu; Zhe Zhang; Zirui Bian

2011

Scholarcy highlights

  • N-acyl-l-homoserine lactones are quorum sensing signal molecules that are commonly used in gram-negative bacteria
  • The cytosolic free Ca2+ concentration was measured by a luminometric method in the excised root cells of Arabidopsis plants that were treated with N-butyryl-homoserine lactone
  • Pretreatments with La3+, verapamil or ethylene glycol tetraacetic acid inhibited the increase incyt caused by C4-HSL, whereas it remained unaffected by pretreatment with Li+, indicating that the Ca2+ contributing to the increase incyt was mobilized from the extracellular medium via the plasma membrane Ca2+ channels but not from the intracellular Ca2+ stores
  • Electrophysiological approaches showed that the transmembrane Ca2+ current was significantly increased with the addition of C4-HSL
  • Our observations suggest that C4-HSL may act as an elicitor from bacteria to plants and that Ca2+ signaling participates in the ability of plant cells to sense the bacterial QS signals
  • ► C4-HSL may act as an elicitor from bacteria in plant. ► Ca2+ signaling participates in the sensing of plant cells to bacterial QS signals. ► C4-HSL can promote a transient elevation incyt

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