Targeted intracellular protein delivery based on hyaluronic acid–green tea catechin nanogels

We developed novel nanogels based on selfassembly of hyaluronic acid –(−)-epigallocatechin-3-gallate conjugates, linear polyethylenemine and the cytotoxic protein Granzyme B for the intracellular delivery of GzmB for cancer therapy

Kun Liang

2016

Scholarcy highlights

  • A novel ternary nanogel based on the self-assembly of hyaluronic acid-epigallocatechin gallate conjugates, linear polyethylenimine and Granzyme B in an aqueous environment was developed for the targeted intracellular delivery of GzmB into cancer cells
  • When CD44-overexpressing HCT-116 colon cancer cells were treated with GzmB-encapsulated HA–EGCG nanogels in vitro, a significant cytotoxic effect was observed
  • Little cytotoxic effect was observed in CD44-deficient cells treated with GzmB-encapsulated HA–EGCG nanogels
  • We developed novel nanogels based on selfassembly of hyaluronic acid–(−)-epigallocatechin-3-gallate conjugates, linear polyethylenemine and the cytotoxic protein Granzyme B for the intracellular delivery of GzmB for cancer therapy
  • HA was exploited for its ability to target CD44 which are overexpressed in many types of cancer cells, while EGCG, the main component of green tea catechins, was chosen for its ability to bind to proteins
  • We believe that the applications of the hyaluronic acid-epigallocatechin gallate conjugates nanogels can be expanded to the intracellular delivery of other cytotoxic protein drugs for cancer therapy

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