A novel fluorescent and electrochemical dual-responsive immunosensor for sensitive and reliable detection of biomarkers based on cation-exchange reaction

The CdSe NCs were successfully functionalized with carboxyl groups, making them effectively conjugate with PAMAM

Yuting Zhao; Xiaoli Cai; Chengzhou Zhu; Haipeng Yang; Dan Du

2019

Scholarcy highlights

  • The CdSe NCs were successfully functionalized with carboxyl groups, making them effectively conjugate with PAMAM
  • To construct such a versatile platform, the model analyte, carcinoembryonic antigen, was captured by magnetic Fe3O4 nanoparticles bound with primary antibodies and recognized by the detection antibodies conjugated complex containing poly(amidoamine), carbon nanotube and carboxyl functionalized CdSe nanocrystals
  • The target CEA was detected based on cation-exchange reaction by adding Ag+ to release thousands of cations Cd2+, which were detected by fluorescence and electrochemistry simultaneously
  • The electrochemical measurement was performed by directly detecting Cd2+ through square wave voltammetry, which displayed an excellent correlation with CEA from 5 pg/mL to 50 ng/mL, with a limit of detection of 1.7 pg/mL
  • The proposed dual-responsive platform holds great promises for biomarkers detection in clinical diagnostics and therapy

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