Glycolipid antigen for use in diagnostic assays for bovine tuberculosis

The results show a low association of PZA resistance and pncA gene mutations in a selected patient group from an highly endemic area

A. Ostyn

2002

Scholarcy highlights

  • The sensitivity and specificity of the assay were investigated using sera of cattle from tuberculosis-free herds and from tuberculosis-infected herds
  • No correlation was found between di-O-acyl trehalose-ELISA and the skin test, nor between DAT-ELISA and interferon-γ with bovine purified protein derivative
  • The antibody titres were not related to cell-mediated immunity
  • The objective of the study was to investigate the correlation between PZA susceptibility and mutations in pncA gene in M. tuberculosis isolates from individuals living in a highly endemic area
  • All PZA resistant isolates had no mutation in the gene coding ribosomal protein S1, which has recently been proposed as alternate target for pyrazinoic acid
  • The results showed that the hydrophilic modification of UF membrane contributed to the fouling control in bovine serum albumin and Escherichia coli bacteria filtration
  • The low sensitivity of ELISA might be attributed to a reciprocal relationship between B-cell proliferation and T-cell protective immunity

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