Estimation of the abundance of the cadmium resistance gene cadA in microbial communities in polluted estuary water

We describe a molecular method for estimating the abundance of the cadA gene, which encodes a Cd2+/ATPase protein transporter, in bacterial DNA extracted from samples of environmental water

Cécile Oger

2002

Scholarcy highlights

  • We describe a molecular method for estimating the abundance of the cadA gene, which encodes a Cd2+/ATPase protein transporter, in bacterial DNA extracted from samples of environmental water
  • We describe the development of this method: the choice of two specific primers, based on the sequences encoding the cadmium binding site and the ion channel domains; the construction of a competitor sequence and assessment of its amplification efficiency; and the estimation of the copy number of the cadA gene
  • This molecular procedure was improved to analyze cadA levels in bacterial DNA extracted from estuary water accidentally contaminated with cadmium
  • The results indicated the prevalence of tetracycline, copper, zinc, cadmium, corresponding TC-resistance genes and HRGs in the three vermicompost samples
  • These results suggested that Sulla coronaria in symbiosis with the consortium of plant-growth-promoting rhizobacteria could be useful in the phytoremediation of cadmium-contaminated soils

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