Evidence for free radical formation during the oxidation of 2′-7′-dichlorofluorescin to the fluorescent dye 2′-7′-dichlorofluorescein by horseradish peroxidase:Possible implications for oxidative stress measurements

The oxidation of 2′-7′-dichlorofluorescin to the fluorescent 2′-7′-dichlorofluorescein by horseradish peroxidase was investigated by fluorescence, absorption, and electron spin resonance spectroscopy

Cristina Rota

2002

Scholarcy highlights

  • The oxidation of 2′-7′-dichlorofluorescin to the fluorescent 2′-7′-dichlorofluorescein by horseradish peroxidase was investigated by fluorescence, absorption, and electron spin resonance spectroscopy
  • DCF fluorescence was still observed when H2O2 was omitted, its intensity was reduced by 50%
  • Spectrophotometric measurements clearly showed that DCFH could be oxidized either by HRP-compound I or HRP-compound II with the obligate generation of the DCF semiquinone free radical
  • This work demonstrates that DCF fluorescence cannot be used reliably to measure O2− in cells because O2− itself is formed during the conversion of DCFH to DCF by peroxidases
  • Evidence for free radical formation during the oxidation of 2′-7′-dichlorofluorescin to the fluorescent dye 2′-7′-dichlorofluorescein by horseradish peroxidase:: Possible implications for oxidative stress measurements

Need more features? Save interactive summary cards to your Scholarcy Library.