Formulation of l-asparaginase-loaded poly(lactide-co-glycolide) nanoparticles: influence of polymer properties on enzyme loading, activity and in vitro release

This paper describes the preparation and characterisation of poly(lactide-co-glycolide) nanoparticles containing the enzyme l-asparaginase. l-Asparaginase was encapsulated in PLG nanospheres using a water-in-oil-in-water solvent evaporation technique

Maria Manuela Gaspar

2002

Scholarcy highlights

  • This paper describes the preparation and characterisation of poly(lactide-co-glycolide) nanoparticles containing the enzyme l-asparaginase. l-Asparaginase was encapsulated in PLG nanospheres using a water-in-oil-in-water solvent evaporation technique
  • Encapsulation efficiency and in vitro release properties of the nanoparticles were affected by the PLG molecular weight
  • The nanoparticles made of PLG with free carboxyl-end groups had a high protein loading and provided a continuous delivery of the active enzyme for 20 days
  • The enzyme loading was lower and no active enzyme was detected in the release medium after a 14-day incubation period when nanoparticles were made of PLG with carboxyl-end groups esterified
  • These results give evidence of the potential of PLG nanospheres for the continuous delivery of l-asparaginase for extended periods of time and show the effect of the PLG chain end-groups in the amount and activity of the enzyme loaded into the nanospheres

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