Identification of an oxygen responsive enhancer element in the glyceraldehyde-3-phosphate dehydrogenase gene

The glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase is induced by hypoxia in endothelial cells

Krista K Graven; Qiang Yu; David Pan; Jill S Roncarati; Harrison W Farber

2002

Scholarcy highlights

  • The glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase is induced by hypoxia in endothelial cells
  • To characterize EC specific hypoxia response elements within the GAPDH gene, we performed transient transfection studies in EC, fibroblasts and smooth muscle cells using portions of the GAPDH promoter linked to a CAT reporter gene
  • Electrophoretic mobility shift assays confirmed inducible EC protein binding to this fragment
  • Mutation of either the hypoxia inducible factor-1-binding site or the flanking sequence resulted in complete loss of function and loss of inducible protein binding
  • The novel HIF-1 flanking sequence required for GAPDH upregulation and the protein(s) that bind to it may be EC specific
  • The novel hypoxia inducible factor-1 flanking sequence required for glyceraldehyde-3-phosphate dehydrogenase upregulation and the protein(s) that bind to it may be endothelial cells specific

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