[24] Nitric oxide assay using hemoglobin method

Hemoglobin, along with related hemoproteins such as myoglobin, can be used for the measurement of nitric oxide

Michael E. Murphy; Eike Noack

2004

Scholarcy highlights

  • Hemoglobin, along with related hemoproteins such as myoglobin, can be used for the measurement of nitric oxide
  • The technique is based on the direct reaction between NO and the oxygenated, ferrous form of hemoglobin, which yields the ferric form, methemoglobin, and nitrate
  • This technique has the following advantages over other methods of NO measurement: all reagents are inexpensive and readily available, no complex techniques are required for sample collection or handling, the only large equipment needed for the basic technique is a spectrophotometer, and the assay is conducted under physiological conditions
  • The chapter discusses the reaction that is the basis for the use of hemoproteins as efficient agents to block the biological effects of NO
  • This reaction is proposed as a basis for the measurement of nitric oxide, using electron spin resonance to detect and quantify the nitrosylhemoprotein product

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