Basic local alignment search tool

The current study aimed to examine the efficiency of ligninolytic enzyme-producing bacterial strain isolated from ameliorated distillery sludge by enrichment method for its bioremediation and detoxification potential of biomethanated distillery effluent

Stephen F. Altschul; Warren Gish; Webb Miller; Eugene W. Myers; David J. Lipman

2007

Scholarcy highlights

  • A new approach to rapid sequence comparison, basic local alignment search tool, directly approximates alignments that optimize a measure of local similarity, the maximal segment pair score
  • The current study aimed to examine the efficiency of ligninolytic enzyme-producing bacterial strain isolated from ameliorated distillery sludge by enrichment method for its bioremediation and detoxification potential of biomethanated distillery effluent
  • Out of nineteen bacterial isolates isolated from distillery sludge, one strain VKDS9, having higher ability towards the removal of effluent’s color, was identified as Bacillus albus by 16S rRNA analysis
  • Our results indicate for the first time that S. calchasi may have a definitive host range in North America that includes at least two raptors, Cooper's hawks and red-tailed hawks, within the family Accipitridae
  • The bacterium showed significant decolourization of effluent up to 83% with subsequent reduction of biochemical oxygen demand, chemical oxygen demand, and other physico-chemical parameters including metallic and non-metallic elements in the presence of glucose and peptone at optimal temperature 37 °C, pH 8.0, and 180 rpm within 144 h
  • Each sample was tested for L. monocytogenes and other Listeria spp.; confirmation was conducted via whole genome sequencing and PCR amplification of the sigB gene, respectively
  • We developed a method named GoFDR for predicting Gene Ontology-based protein functions

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