The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen

The reduction of nitro blue tetrazolium with NADH mediated by phenazine methosulfate under aerobic conditions was inhibited upon addition of superoxide dismutase. This observation indicated the involvement of superoxide aninon radical in the reduction of NitroBT, the radical being generated in the reoxidation of reduced PMS

Morimitsu Nishikimi; N. Appaji Rao; Kunio Yagi

2005

Scholarcy highlights

  • The reduction of nitro blue tetrazolium with NADH mediated by phenazine methosulfate under aerobic conditions was inhibited upon addition of superoxide dismutase. This observation indicated the involvement of superoxide aninon radical in the reduction of NitroBT, the radical being generated in the reoxidation of reduced PMS
  • A simple method for detecting superoxide dismutase is described

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