Observation of terahertz vibrations in Pyrococcus furiosus rubredoxin via impulsive coherent vibrational spectroscopy and nuclear resonance vibrational spectroscopy – interpretation by molecular mechanics

We have used impulsive coherent vibrational spectroscopy to study the Fe(S-Cys)4 site in oxidized rubredoxin from Pyrococcus furiosus

Ming-Liang Tan

2006

Scholarcy highlights

  • We have used impulsive coherent vibrational spectroscopy to study the Fe(S-Cys)4 site in oxidized rubredoxin from Pyrococcus furiosus
  • A 15 fs visible laser pulse is used to coherently pump the sample to an excited electronic state, and a second <10 fs pulse is used to probe the change in transmission as a function of the time delay
  • PfRd was observed to relax to the ground state by a single exponential decay with time constants of ∼255–275 fs
  • Superimposed on this relaxation are oscillations caused by coherent excitation of vibrational modes in both excited and ground electronic states
  • Fourier transformation reveals the frequencies of these modes
  • The lowest frequency normal modes are identified as collective motions of the entire protein or large segments of polypeptide. Motion in these modes may affect the polar environment of the redox site and tune the electron transfer functions in rubredoxins

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