The Aedes aegypti glutathione transferase family

We describe the glutathione transferase gene family in the dengue vector Aedes aegypti and suggest a novel role for a new class of mosquito Glutathione S-transferases

Nongkran Lumjuan; Bradley J. Stevenson; La-aied Prapanthadara; Pradya Somboon; Peter M. Brophy; Brendan J. Loftus; David W. Severson; Hilary Ranson


Scholarcy highlights

  • Glutathione S-transferases are major phase II detoxification enzymes found in most organisms
  • Twenty-six GST genes are present in Ae. aegypti, two of which are alternatively spliced to give a total of 29 transcripts for cytosolic GSTs
  • The six classes identified in other insect species are all represented and, as in Anopheles gambiae, the majority of the mosquito GSTs belong to the insect-specific Delta and Epsilon classes with eight members each
  • The GST supergene family is surprisingly conserved between Ae. aegypti and An. gambiae
  • Many clear orthologs can be identified between the two species and in both Aedes and Anopheles two GST genes are alternatively spliced to create additional diversity in this enzyme family
  • The Delta and Epsilon classes, both specific to insects, are the largest classes comprising over 65% of the total complement of cytosolic GSTs in these two Diptera
  • The major difference is in the representation of the GST classes, with more Theta class GSTs and fewer Delta GSTs in Ae. aegypti compared to An. gambiae
  • The mRNA level of the BgGSTD1 gene in the fat body of B. germanica peaked early in the day or subjective day under light-dark or constant dark conditions, whereas enzyme activity of cytosolic Glutathione S-transferases did not reflect the rhythmic pattern as well as BgGSTD1 expression

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