Differential expression patterns of two new primary cell wall-related cellulose synthase cDNAs, PtrCesA6 and PtrCesA7 from aspen trees

We report on cloning of two new cellulose synthase cDNAs, PtrCesA6 and PtrCesA7 involved in the primary cell wall development in aspen trees

Anita Samuga; Chandrashekhar P Joshi

2004

Scholarcy highlights

  • Cellulose is an integral component of plant cell walls
  • We report on cloning of two new cellulose synthase cDNAs, PtrCesA6 and PtrCesA7 involved in the primary cell wall development in aspen trees
  • PtrCESA7 shares a maximum identity of 86% with AtCESA2, a primary wall-related CesA member from Arabidopsis, a dicot species
  • In situ mRNA hybridization studies using three different aspen organs suggested that PtrCesA6 and PtrCesA7 genes are expressed in all expanding cells depositing primary cell wall but PtrCesA7 is expressed at a higher level than PtrCesA6
  • AtCesA2 gene expression is shown in primary wall enriched tissues but AtCesA2 antisense plants showed weak mutant phenotype suggesting that AtCesA2 performs some redundant function in primary cell wall development
  • Root volume tolerance index significantly increased in Coker 312 at 1000 μM Cd
  • Our results for cDNA cloning, expression analyses, and phylogenetic analysis suggest that the 10 BoCesA genes cloned from the etiolated bamboo shoots participate in cellulose synthesis in the primary cell walls of the growing bamboo, and that at least three additional BoCesA genes involved in cellulose synthesis in the secondary walls may be present in the bamboo genome

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