Reversed chitosan–alginate polyelectrolyte complex for stability improvement of alpha-amylase: Optimization and physicochemical characterization

The present work explores, using response surface methodology, the main and interaction effects of some process variables on the preparation of a reversed chitosan–alginate polyelectrolyte complex with entrapped α-amylase for stability improvement

Mayur G. Sankalia

2006

Scholarcy highlights

  • The present work explores, using response surface methodology, the main and interaction effects of some process variables on the preparation of a reversed chitosan–alginate polyelectrolyte complex with entrapped α-amylase for stability improvement
  • A 33 full factorial design was used to investigate the effect of the chitosan and alginate concentrations and hardening time on the percent entrapment, time required for 50% and 90% enzyme release, and particle size
  • The in vitro enzyme release profile of the beads was fitted to various release kinetics models to study the release mechanism
  • Beads prepared using 2.5% w/v chitosan and 3% w/v sodium alginate with a hardening time of 60 min had more than 90% entrapment and a T90 value greater than 48 min
  • Proper selection of the reaction pH, polymer concentration and charge density, and hardening time is important and determines the characteristics of the polyelectrolyte complex

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