The structure at 2 Å resolution of Phycocyanin from Gracilaria chilensis and the energy transfer network in a PC–PC complex

This paper reports the three dimensional structure of phycocyanin from G. chilensis, the building of a docking model of two PC hexamers refined by molecular dynamics and the determination of the constants for the energy transfer in resonance between pairs of chromophores

Carlos Contreras-Martel

2006

Scholarcy highlights

  • Phycobilisomes are auxiliary photosynthetic complexes present in cyanobacteria and eukaryotic red algae
  • This paper reports the three dimensional structure of phycocyanin from G. chilensis, the building of a docking model of two PC hexamers refined by molecular dynamics and the determination of the constants for the energy transfer in resonance between pairs of chromophores
  • Phycocyanin from G. chilensis was purified according to Gantt
  • Other phycobiliproteins are purified along with associated linker polypeptides, SDS-PAGE analysis of the purified phycocyanin of G. chilensis showed no presence of other polypeptides besides the α and β subunits
  • Energy transfer steps for all chromophore pairs in our system were calculated and the value of the inverse of the transfer constant expressed in ps for the intra- or inter-phycocyanin steps are shown on Table 2
  • The structure allowed to build a reliable model of the PC–PC complex, a minimum unit of a rod in phycobilisomes and to
  • As the asymmetric unit was the hexamer, it was possible to detect slight differences between equivalent subunits, representing more accurately the biological functional unit

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