Electrochemically deposited chitosan hydrogel for horseradish peroxidase immobilization through gold nanoparticles self-assembly

A new strategy for immobilization of horseradish peroxidase has been presented by self-assembling gold nanoparticles on chitosan hydrogel modified Au electrode

Xi-Liang Luo; Jing-Juan Xu; Qing Zhang; Gong-Jun Yang; Hong-Yuan Chen

2004

Scholarcy highlights

  • A new strategy for immobilization of horseradish peroxidase has been presented by self-assembling gold nanoparticles on chitosan hydrogel modified Au electrode
  • From a mildly acidic chitosan solution, a chitosan film is electrochemically deposited on Au electrode surface via a negative voltage bias
  • This process is accompanied by the hydrogen evolution reaction, and the released hydrogen gas made the deposited chitosan film with porous structure, which facilitates the assembly of gold nanoparticles and HRP
  • The resulting substrates were characterized by atomic force microscopy and electrochemical impedance spectroscopy
  • The biosensor remained about 85% of its original sensitivity after four weeks’ storage
  • The resulting biosensor showed a wide dynamic range of 8.0 μM–15 mM H2O2, and the linear ranges were 8.0 μM–0.12 mM and 0.50–12 mM, with a detection limit of 2.4 μM estimated at a signal-to-noise ratio of 3

Need more features? Save interactive summary cards to your Scholarcy Library.