The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells

We find that when DNA breakage resulting from H2O2 treatment is examined, freshly isolated normal human lymphocytes are relatively resistant compared with transformed human cells

Andrew R. Collins; Ma Ai-guo; Susan J. Duthie

2002

Scholarcy highlights

  • Single cell gel electrophoresis is a sensitive method for detecting DNA strand breaks
  • Cells embedded in agarose are converted to nucleoids by treating with detergent and high salt
  • We find that when DNA breakage resulting from H2O2 treatment is examined, freshly isolated normal human lymphocytes are relatively resistant compared with transformed human cells
  • While the addition of deoxyribonucleosides promotes completion of repair of UV damage by lymphocytes, it has no significant effect on repair of oxidative damage

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