Decreased production of reactive oxygen intermediates is an early event during in vitro apoptosis of rat thymocytes

The results suggest that a decreased spontaneous production of reactive oxygen intermediates in thymocytes precedes the spontaneous in vitro apoptosis and Dex exaggerates these changes

Ji-Feng Wang; Thomas R. Jerrells; John J. Spitzer

2002

Scholarcy highlights

  • Thymocyte apoptosis is one of the best characterized experimental models of apoptosis that can be induced by a variety of stimuli such as glucocorticoids, ionizing radiation, antibodies, and toxins
  • Little is known about the production and possible function of reactive oxygen intermediates in thymocytes
  • Significant inhibition of the intracellular fluorescence was observed by addition of N-nitro-l-arginine methyl ester, that could not be reversed by L-arginine
  • The addition of N-nitro-D-arginine methyl ester caused considerable inhibition. This indicates that the inhibition by L-NAME or D-NAME is due to a direct scavenging effect, and nitric oxide production is not likely to be involved
  • The ROI production was decreased with time after incubation of the thymocytes for 1, 3, and 6 h in vitro
  • The results suggest that a decreased spontaneous production of ROI in thymocytes precedes the spontaneous in vitro apoptosis and Dex exaggerates these changes

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