Study of the mechanism of interaction of poly(ϵ-caprolactone) nanocapsules with the cornea by confocal laser scanning microscopy

With the aim of exploring the potential of poly(ϵ-caprolactone) nanocapsules as drug carriers for ocular administration, the present study examined the mechanism of interaction of these nanocapsules with the corneal and conjunctival epithelia

P CALVO

2002

Scholarcy highlights

  • With the aim of exploring the potential of poly(ϵ-caprolactone) nanocapsules as drug carriers for ocular administration, the present study examined the mechanism of interaction of these nanocapsules with the corneal and conjunctival epithelia
  • In the first stage of this work, corneas were mounted in a perfusion cell, incubated with a suspension of rhodamine 6G-loaded PECL nanocapsules and subsequently observed by confocal laser scanning microscopy
  • Fluorescence signals were only observed within the epithelial cells, but not in the intercellular junctions, demonstrating the intracellular localization of the fluorescent nanocapsules
  • To determine whether this penetration could be associated with cellular lysis or endocytotic uptake, corneas were pretreated with blank nanocapsules and stained with propidium iodide, a fluorescent dye which distinguishes viable from non-viable cells
  • No nanocapsules were observed in the conjunctival epithelium, which indicates a selective interaction of the PECL nanocapsules for the cornea vs the conjunctiva
  • This implies a potential increase in therapeutic effect and a reduction of systemic side effects

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