The use of synthetic oligodeoxyribonucleotides to produce specific deletions in the araBAD promoter of Escherichia coli B/r

Two oligodeoxyribonucleotides were chemically synthesized and used to mutate the regulatory region of the araBAD operon in Escherichia coli B/r

C.Garrett Miyada

2004

Scholarcy highlights

  • Two oligodeoxyribonucleotides were chemically synthesized and used to mutate the regulatory region of the araBAD operon in Escherichia coli B/r
  • The mutations were introduced onto an ara insert cloned in an M13 vector using the synthetic oligodeoxyribonucleotides as primers and the strand of an M13 mp2::ara hybrid phage as a template in an in vitro polymerization reaction
  • The mutant ara inserts were subcloned into a stable plasmid for functional analysis
  • Transcription studies performed on strains containing the mutant ara plasmids demonstrated that both mutations reduced the amount of araBA mRNA synthesized in the presence of l-arabinose

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