Plant regeneration via somatic embryogenesis in mature embryo-derived callus culture of Sinocalamus latiflora (Munro) McClure

Somatic embryogenesis and subsequent formation of plantlets was achieved from callus cultures derived from mature zygotic embryos of Sinocalamus latiflora McClure

Meei-Ling Yeh

2003

Scholarcy highlights

  • Somatic embryogenesis and subsequent formation of plantlets was achieved from callus cultures derived from mature zygotic embryos of Sinocalamus latiflora McClure
  • Embryogenic callus was initiated on Murashige and Skoog's medium supplemented with 6 mg/l 2,4-dichlorophenoxyacetic acid, 3 mg/l kinetin, 250 mg/l polyvinylpyrrolidon and 5% sucrose
  • Prolonged culture of the embryogenic callus on the same medium resulted in embryoid formation
  • The embryoids developed further to yield whole plantlets when transferred to a medium containing lower concentrations of 2,4-D and kinetin

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