Gas chromatography-mass spectrometry methods for analysis of 2- and 3-hydroxylated fatty acids: Application for endotoxin measurement

Our results show that both the ratio of the summed iso and anteiso to the total amount of normal 3-OH-fatty acid, and the ratio of summed iso and anteiso to the total amount of all 3-OH-FAs were primarily related to the pH of soil

Zbigniew Mielniczuk

2002

Scholarcy highlights

  • Gas chromatography-mass spectrometry methods for analysis of 2- and 3-hydroxy fatty acids were evaluated
  • Both 2- and 3-hydroxylated acids with 10–18 carbon atoms were detected in organic dust collected from a ventilation duct; differential ion monitoring was used for their separation
  • The FA composition of Xanthomonas campestris pathovar campestris, Xanthomonas perforans, Clavibacter michiganensis subsp. michiganensis and Streptomyces scabies were dominated by iso- and anteiso-fatty acids, which is typical for most bacteria
  • Our new field-based correlations demonstrate the physiological response of Gram-negative bacterial cell membranes to the external environment and suggest that 3-hydroxy fatty acids can be applied in palaeoenvironmental studies to estimate past mean annual air temperature and soil pH
  • No generalization between the total and bioactive endotoxins can be drawn from the study, the levels reported in this study suggests that the discrepancies between the two measurement approaches, and the bioactive potential of 3-OHFAs with individual carbon chains deserve further investigation
  • The maximum NO reduction efficiency of 82.70% and 78.52% can be obtained at 1323 K, respectively
  • To evaluate the flow characteristics with respect to the overfire air angle and establish an optimal OFA angle for the furnace, cold airflow experiments were conducted by recording flow field data within a 1:15-scaled model of the furnace at different OFA angle settings
  • Our published numerical results uncovered that applying the deep-air-staging combustion technology with the optimized overfire air angle, well-formed symmetric combustion developed and NOx emissions could be reduced by 50%, without increasing levels of carbon in fly ash

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