Comparison of enteric Adenovirus infection in various human cell lines

The results indicate the distinct replication characteristics exhibited by adenovirus 40 are not due to a restriction in a specific host cell, and, because purified virions were used, not attributable to interference that might occur with co-infection from multiple viruses present in the same clinical specimen

Donald J. Witt; Estelle B. Bousquet

2002

Scholarcy highlights

  • Growth of the fastidious enteric adenoviruses 40 and 41 was compared in different human cell lines
  • Low infectious titers were obtained from each cell type following infection with adenovirus, whereas adenovirus replicated to significantly higher titer
  • A time course experiment performed to quantitate the amount of hexon present in A549 and KB cells infected with each virus indicated that while the kinetics of accumulation were similar for both viruses, the concentration of type 41 hexon was significantly greater than that for type in either cell line
  • The concentration of type hexon was similar in each cell type; for type 40, a greater concentration of hexon was obtained in the A549 cell line than in the KB cells
  • The results indicate the distinct replication characteristics exhibited by adenovirus 40 are not due to a restriction in a specific host cell, and, because purified virions were used, not attributable to interference that might occur with co-infection from multiple viruses present in the same clinical specimen
  • We conclude the differences observed in the replication of these viruses are independent of host cell type and are associated, uniquely, with each virus

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