Fibrinogen structure in projection at 18 Å resolution

Electron microscope images of frozen-hydrated crystals of a proteolytically modified fibrinogen show excellent preservation of the structure

S.P.Sudhakara Rao

2004

Scholarcy highlights

  • Electron microscope images of frozen-hydrated crystals of a proteolytically modified fibrinogen show excellent preservation of the structure
  • An electron density map of the key centric projection of the crystal at 18 Å resolution has been obtained by combining the phases derived from cryo-electron microscopy with X-ray amplitudes
  • Some additional constraints useful for the determination of the three-dimensional structure were obtained from cryo-electron micrographs of a perpendicular view at 25 Å resolution
  • The data used here will be valuable as a starting point for obtaining the three-dimensional structure
  • Funds to purchase and maintain the computer system were obtained from a shared instrumentation grant 1-S10-RR04671-01 awarded to David J
  • Funds to purchase and maintain the Philips EM420T transmission electron microscope were obtained from a shared instrumentation grant 1-S10-RR02464-01 awarded to C.C. by the National Institutes of Health

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