Development and regulation of substance P in sensory neurons in vitro

Using different culture techniques and a sensitive radioimmunoassay for Substance P, we studied the ontogeny and regulation of this functionally important neurotransmitter in these neurons, obtained from neonatal rats

Joshua E. Adler

2005

Scholarcy highlights

  • Substance P, the putative neuropeptide mediator of pain sensation, is contained in small dorsomedial sensory neurons of the dorsal root ganglion
  • This rise was predominantly due to mechanisms intrinsic to the ganglion since it occurred in a fully defined medium, in the absence of added nerve growth factor
  • Depolarization with veratridine blocked the increase in SP, an effect which was reversed by tetrodotoxin, suggesting that transmitter characteristics in sensory neurons may be regulated by depolarization and/or transmembrane sodium flux
  • Raising the dose of the trophic factor had no incremental effect on SP content, suggesting that NGF was acting primarily on neuronal survival
  • Veratridine blocked the increase in SP in a tetrodotoxin-reversible manner, without affecting neuronal survival, indicating that the effects of these agents do not depend on normal ganglionic cellular architecture
  • Our studies suggest that the development of transmitter characteristics in primary sensory neurons may be regulated by multiple factors, including neuronal activity as well as trophic agents such as NGF
  • Whittier Fellowship from the Committee to Combat Huntington's Disease

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