Some observations on the proteins of the inner root sheath cells of hair follicles

A method is described by which the inner root sheaths of hair follicles can be obtained intact and in quantities sufficient for study by light microscopy, X-ray diffraction and paper chromatography

G.E. Rogers

2003

Scholarcy highlights

  • A method is described by which the inner root sheaths of hair follicles can be obtained intact and in quantities sufficient for study by light microscopy, X-ray diffraction and paper chromatography
  • The cells of the inner root sheat initially contain granules of trichohyalin which are later replaced by fibrous material; it is shown that both are protein in nature
  • It is shown that the fibrous protein which is the major component of the inner root sheath, is rapidly digested by proteolytic enzymes whereas the cell membranes and nuclei are resistant to attack
  • Analysis of hydrolysates of inner root sheaths by paper chromatography reveal that the fibrous protein has an amino acid composition markedly different from that of hair
  • Most important of all is the presence of citrulline in the fibrous protein. This result constitutes the first demonstration of citrulline in a protein. It is absent from the protein derived from trichohyalin granules
  • The significance of the presence of citrulline is discussed in terms of its possible influence on the properties of the protein and its role in follicle metabolism

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