Paraquat-induced chemiluminescence of microsomal fractions

Selective light emission at 634 and 703 nm with a minimum intensity at 668 nm points to the involvement of singlet molecular oxygen in the paraquat-elicited photoemission

Enrique Cadenas; Regina Brigelius; Helmut Sies

2002

Scholarcy highlights

  • Selective light emission at 634 and 703 nm with a minimum intensity at 668 nm points to the involvement of singlet molecular oxygen in the paraquat-elicited photoemission
  • Paraquat-dependent chemiluminescence was not associated with accumulation of malondialdehyde, and addition of the bipyridylium compound to an ongoing lipid peroxidation reaction produced a different time course for this chemiluminescence and inhibited malondialdehyde formation
  • Accumulation of the paraquat radical under anaerobiosis was not accompanied by chemiluminescence, but subsequent oxygenation of the sample produced a burst of chemiluminescence, the intensity of which was related to the time of incubation under anaerobiosis and paraquat radical accumulated
  • Superoxide dismutase inhibits this chemiluminescence, suggesting the involvement of O2тик; catalase was without effect
  • At variance with the microsomal fraction, paraquat-infused perfused liver did not show any noticeable chemiluminescence, probably due to the presence of cellular defense mechanisms

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