Role of multiple binding sites in the inhibition of NADH oxidase by piericidin and rotenone

Scatchard plots indicate that 14C-piericidin A and -rotenone bind at 2 specific sites per mole of NADH dehydrogenase in ETP, but the titer found for complex I or mersalyl-treated ETP more closely approximates 1

M. Gutman; Thomas P. Singer; John E. Casida

2004

Scholarcy highlights

  • Scatchard plots indicate that 14C-piericidin A and -rotenone bind at 2 specific sites per mole of NADH dehydrogenase in ETP, but the titer found for complex I or mersalyl-treated ETP more closely approximates 1
  • The curves for inhibition of NADH oxidase by piericidin and rotenone are sigmoidal; this results from an unequal contribution of the 2 specific sites to the inhibition
  • Inhibition of NADH-CoQ reductase activity is due entirely to binding at specific site(s) because bovine serum albumin does not restore activity

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