ADP-iron as a fenton reactant: Radical reactions detected by spin trapping, hydrogen abstraction, and aromatic hydroxylation

A mixture of ADP, ferrous ions, and hydrogen peroxide generates hydroxyl radicals that attack the spin trap DMPO to yield the hydroxyl free radical spin-adduct, degrade deoxyribose and benzoate with the release of thiobarbituric acid-reactive material, and hydroxylate benzoate to give fluorescent products

John M.C. Gutteridge

2004

Scholarcy highlights

  • A mixture of ADP, ferrous ions, and hydrogen peroxide generates hydroxyl radicals that attack the spin trap DMPO to yield the hydroxyl free radical spin-adduct, degrade deoxyribose and benzoate with the release of thiobarbituric acid-reactive material, and hydroxylate benzoate to give fluorescent products
  • Inhibition studies, with scavengers of the .OH radical, suggest that the behavior of iron-ADP in the reaction is complicated by the formation of ternary complexes with certain scavengers and detector molecules
  • Under certain reaction conditions the ligand may be the major recipient of .OH radical damage thereby leading to the incorrect assumption that the iron ligand is a poor Fenton reactant

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