The role of H2O2 generation in perfused rat liver and the reaction of catalase compound I and hydrogen donors

The results strongly suggest that one of the major sources for H2O2 production is the mitochondrial system participating in fatty acid oxidation

Nozomu Oshino

2004

Key concepts

Scholarcy highlights

  • The method is based on a relationship between the ratio of H2O2 generaztion rate to catalase-heme concentration and the concentration of methanol which causes a decrease in the pme value to half of its saturation value
  • Endogenous hydrogen donor in the perfused liver was estimated to be equivalent to about 30 μm methanol
  • Mitochondrial production of H2O2 was proved by the fact that fatty acids enhance the H2O2 production and that this enhancement was inhibited by either rotenone or antimycin A
  • In the presence of 0.3 mm octanoate, H2O2 generation increased to 170 nmol/min/g wet wt liver
  • The rate of H2O2 production in the perfused liver with 2 mml-lactate and 0.3 mm pyruvate was 49 nmol/min/g wet wt liver at 30 °C. This rate corresponds to about 1.7% of the total liver respiration rate. 3
  • The results strongly suggest that one of the major sources for H2O2 production is the mitochondrial system participating in fatty acid oxidation
  • The general conclusion is that, the body regulates the intracellular H2O2 level below about 10−7m, and with this level biological oxidations of considerable significance are possible through the catalase system

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