Improved in vitro shoot multiplication and rooting of Dendrocalamus hamiltonii Nees et Arn. Ex Munro: production of genetically uniform plants and field evaluation

An efficient in vitro regeneration protocol and field performance of a multipurpose bamboo species Dendrocalamus hamiltonii Nees et Arn

Rajneesh K. Agnihotri; Janhvi Mishra; Shyamal Kumar Nandi

2009

Scholarcy highlights

  • An efficient in vitro regeneration protocol and field performance of a multipurpose bamboo species Dendrocalamus hamiltonii Nees et Arn
  • Ex Munro has been demonstrated using single node cuttings taken from the lateral branches of a 20-year-old bush
  • Axillary buds on the nodal explant sprouted within 10 days of culture on Murashige and Skoog medium without any plant growth substance
  • High-frequency proliferation was induced on the propagules
  • A multiplication of about 20-folds was achieved on MS medium supplemented with 8 μM BAP and 1 μM NAA
  • Rooting efficiency was markedly enhanced when the propagules, following shoot cut, were placed on to MS medium supplemented with 100 μM IBA for 10 days and transferred to IBA-free medium
  • Genetic fidelity of these regenerants was established by RAPD analysis advocating clonal propagation of this species through nodal segment culture and its commercial cultivation

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