Identification and characterization of microRNAs from citrus expressed sequence tags

We present a study of bioinformatics identification of microRNA precursors in citrus by comparing known plant miRNAs against 560,271 citrus ESTs

Xiao-Meng Wu

2010

Scholarcy highlights

  • MicroRNAs are a group of single-stranded noncoding RNAs with general size of 21–24 nucleotides, which negatively regulate gene expression posttranscriptionally by repressing gene translation or degrading targeted mRNAs
  • Despite the important functions of miRNAs in plants, little is known about miRNAs in citrus
  • We present a study of bioinformatics identification of microRNA precursors in citrus by comparing known plant miRNAs against 560,271 citrus ESTs
  • Seventy-eight ESTs from ten citrus species and three hybrids were identified as putative miRNA precursors, encoding 51 unique miRNA sequences, representing 28 miRNA families
  • 36 unigenes and 77 ESTs were identified as putative targets of 25 citrus miRNA families
  • The cleavage sites on five mRNAs targeted by three miRNA families were mapped by 5′RACE
  • This study provided information on citrus miRNA precursors, mature miRNAs, and miRNA targets and is helpful for future research of miRNA function in citrus

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