Somatic embryogenesis and organogenesis in Dendrocalamus hamiltonii

The results showed that the genotypic variances in general and specific combining abilities of callus induction frequencies and shoot differentiation frequencies were significant at the 1% level

Ning Zhang; Wei Fang; Yan Shi; Qianqian Liu; Haiyun Yang; Renyi Gui; Xinchun Lin

2010

Scholarcy highlights

  • In this study, mature zygotic embryos, plant growth regulators, and various media were tested with the aim of developing an efficient regeneration system for plantlets of the bamboo species Dendrocalamus hamiltonii
  • The induction basal medium for anther culture was NB medium, while Murashige and Skoog basal medium was used for shoot differentiation
  • The results showed that the genotypic variances in general and specific combining abilities of callus induction frequencies and shoot differentiation frequencies were significant at the 1% level
  • The effect of HB medium supplemented with 2 mg/l 2,4-D on callus induction and maintenance in 25 wheat genotypes in comparison with that of N6 medium
  • A few calli induced from spikelets germinated, whereas calli induced from seeds had a regeneration rate of up to 80%
  • The results showed that HB medium was more effective in terms of both callus induction and maintenance
  • The Research Center for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, 88 North Circular Road, 311300, Lin’an, Zhejiang, People’s Republic of China

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