Comparison of maize brown-midrib isogenic lines by cellular UV-microspectrophotometry and comparative transcript profiling

The molecular mechanisms underlying cell wall digestibility in maize have been studied in three sets of maize brown-midrib isogenic lines in the genetic background of inbreds 1332, 5361, and F2

Chun Shi; Gerald Koch; Milena Ouzunova; Gerhard Wenzel; Imad Zein; Thomas Lübberstedt

2006

Scholarcy highlights

  • The molecular mechanisms underlying cell wall digestibility in maize have been studied in three sets of maize brown-midrib isogenic lines in the genetic background of inbreds 1332, 5361, and F2
  • 53 ESTs were differentially expressed in all three isogenic bm3 comparisons, whereas 32 ESTs were consistently differentially expressed in different bm isogenic lines in F2 background
  • About 70% ESTs isolated by SSH were not present on the unigene microarray, demonstrating the usefulness of the SSH procedure to identify genes related to cell wall digestibility
  • The down-regulation of COMT affected the expression of CCoAOMT genes and caused a reduced content both of G and S units in bm3 mutants
  • Per Gregersen, and Christina Ingvardsen for assistance with microarray experiments in Research Centre Flakkebjerg

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