A Method to Pre-Screen Rat Mammary Gland Whole-Mounts Prior To RNAscope

RNAscope is a quantitative in situ gene expression measurement technique that preserves the spatial aspect of intact tissue; allowing for comparison of specific cell populations and morphologies

Emily L. Duderstadt; Mary Ann Sanders; David J. Samuelson

2021

Scholarcy highlights

  • RNAscope is a quantitative in situ gene expression measurement technique that preserves the spatial aspect of intact tissue; allowing for comparison of specific cell populations and morphologies
  • Reliable and accurate measurement of gene expression in tissue is dependent on preserving RNA integrity and the quantitative nature of RNAscope
  • The purpose of this study was to determine if the quantitative nature of RNAscope was retained following processing and carmine staining of mammary gland whole-mounts, which are commonly used to identify lesions, such as hyperplasia and ductal carcinoma in situ
  • We were concerned that handling and procedures required to visualize microscopic disease lesions might compromise RNA integrity and the robustness of RNAscope
  • This was determined in comparison to tissue that had been formalin-fixed and paraffin embedded immediately after collection
  • & Samuelson, D.J. A Method to Pre-Screen Rat Mammary Gland Whole-Mounts Prior To RNAscope

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