A Detailed Mammosphere Assay Protocol for the Quantification of Breast Stem Cell Activity

We describe here a detailed mammosphere assay protocol for the assessment of stem cell activity and self-renewal, and discuss how data generated by the assay can be analysed and interpreted

Frances L. Shaw; Hannah Harrison; Katherine Spence; Matthew P. Ablett; Bruno M. Simões; Gillian Farnie; Robert B. Clarke

2012

Scholarcy highlights

  • Since the discovery that neural tissue contains a population of stem cells that form neurospheres in vitro, sphere-forming assays have been adapted for use with a number of different tissue types for the quantification of stem cell activity and self-renewal
  • One tissue type widely used for stem cell investigations is mammary tissue, and the mammosphere assay has been used in both normal tissue and cancer
  • We describe here a detailed mammosphere assay protocol for the assessment of stem cell activity and self-renewal, and discuss how data generated by the assay can be analysed and interpreted

Need more features? Save interactive summary cards to your Scholarcy Library.