Optimization of macrophage isolation from the Persian sturgeon and the Caspian kutum fish: a comparative study

The results showed that the

Sara Rastgar; Abdolali Movahedinia; Negin Salamat; Amirparviz Salati; Ebrahim Zabihi

2018

Scholarcy highlights

  • Fish, as a diverse group of vertebrates, can be used extensively as a biological model system
  • Some contaminating non-adherent small cells; presumably lymphocytes were observed in the macrophage culture but vanished after washing the cultured macrophages
  • The present study successfully optimized macrophage primary culture for two fish species from two different bony fish orders, and these methods can be used for future study on these cells and fish immune system
  • The results showed that the Caspian kutum macrophages were more influenced by seeding cell density, adherence, incubation time and culture temperature whereas the macrophages from the Persian sturgeon were more sensitive to pH of the culture medium
  • Both fish macrophages responded to the natural light cycle by increasing reactive oxygen species and nitric oxide
  • The finding related to the effect of visible light on fish macrophages is novel and may be useful for studies on how light may affect fish biological systems especially the immune system

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