In vitro metastatic colonization of human ovarian cancer cells to the omentum

We describe a technique for identifying, isolating and maintaining ex vivo cultures of omenta from immune-compromised and -competent mice

Shaheena M. Khan

2010

Scholarcy highlights

  • Despite the potentially crucial contributions of the omentum in the regulation of ovarian cancer metastatic growth, it remains a poorly understood organ
  • Further experiments demonstrate that the ex vivo culture conditions allow for the proliferation of ovarian cancer cells in vitro and support a similar pattern of microscopic lesions after either intraperitoneal injection of ovarian cancer cells or co-culture of ovarian cancer cells with the omentum
  • In agreement with previous studies from our laboratory, histologic evaluation of these specimens found that ovarian cancer cells, as well as other peritoneal cancer cells, preferentially accumulate in, and colonize, omental areas rich in immune cells
  • This work was made possible by the generous philanthropic support of The University of Chicago Section of Urology and grants from The Department of Defense Ovarian Cancer Research Program and the National Cancer Institute

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