Heterologous expression of a novel Poa pratensis gibberellin 2-oxidase gene, PpGA2ox, caused dwarfism, late flowering, and increased chlorophyll accumulation in Arabidopsis

Our study suggests that PpGA2ox could be a candidate gene for breeding new cultivars of P. pratensis

P.-H Tan; L. Zhang; S.-X. Yin; K. Teng

2018

Scholarcy highlights

  • Bioactive gibberellins play crucial roles in plant growth and development including stem elongation, leaf expansion, and flower development
  • The PpGA2ox cDNA sequence was deposited in the NCBI database with the accession number of KX254272
  • Phylogenetic analysis showed that PpGA2ox was most closely related to the proteins from Hordeum vulgare or Triticum aestivum
  • The results showed that the expression of the selected marker genes related to gibberellin catabolism were higher in the transgenic lines than in the WT plants
  • PpGA2ox belonged to the GA2ox superfamily and contained three Fe2+ binding sites and one 2-oxoglutarate binding site. It was localized in both nucleus and cytoplasm which was consistent with the localization AtGA2ox4 in Arabidopsis, OsGA2ox6 in rice, and
  • Previous studies proved that GA2oxs play diverse roles in regulating gibberellin metabolism compared with the cytosol localized GA20ox and GA3ox
  • Overexpression of PpGA2ox caused GA-deficient phenotype in Arabidopsis including dwarf plants, delayed flowering time, and increased chlorophyll content

Need more features? Save interactive summary cards to your Scholarcy Library.