Effect of advanced glycation endproducts on gene expression profiles of human dermal fibroblasts

We studied the effect of two AGE-products prepared by the glycation of lysozyme and of BSA, on the expression profile of a large number of genes potentially involved in the above mentioned effects of AGE-s

J. Molinari; E. Ruszova; V. Velebny; L. Robert

2008

Scholarcy highlights

  • The Maillard reaction and its end products, AGE-s are rightly considered as one of the important mechanisms of post-translational tissue modifications with aging
  • We studied the effect of two AGE-products prepared by the glycation of lysozyme and of BSA, on the expression profile of a large number of genes potentially involved in the above mentioned effects of AGE-s
  • The two AGE-products were added to human skin fibroblasts and gene expression profiles investigated using microarrays
  • Among the large number of genes monitored the expression of 16 genes was modified by each AGE-preparations, half of them only by both of them
  • The lysozyme-AGE preparation upregulated selectively the genes coding for acid phosphatase, integrin chain α5 and thrombospondin which were unaffected by the BSA-AGE preparation
  • It was shown previously that the lysozyme-AGE strongly increased the rate of proliferation and cell death, much more than the BSA-AGE preparation. These differences between these two AGE-preparations tested suggest the possibility of different receptor-mediated transmission pathways activated by these two preparations
  • Most of the gene-expression modifications are in agreement with biological effects of Maillard products, especially interference with normal tissue structure and increased tissue destruction

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