Sequence identification and expression profile of seven Dermacentor marginatus glutathione S-transferase genes

We found there were linear B-cell epitopes in different D. marginatus glutathione S-transferase shared similar prediction patterns suggesting that they might have cross immunity within GST classes

Huercha; Ying Ma; Yunwei Hao; Min Li; Zhengxiang Hu; Ruiqi Song; Liting Wei; Shilong Fan; Songqin Chen; Xinli Fan; Xuejie Zhai; Qingyong Guo; Chahan Bayin

2020

Scholarcy highlights

  • Ticks are obligate hematophagous ectoparasites of great medical and veterinary importance
  • Full coding regions corresponding to D. marginatus glutathione S-transferase were obtained by real-time quantitative PCR) at annealing temperatures and extension durations according to amplification conditions
  • The phylogenetic tree was built by PhyML, and phylogenetic analysis demonstrated the grouping of the seven GSTs in various clades, respectively
  • The following conclusions can be drawn from the present study that among the seven D. marginatus glutathione S-transferases, mu-class GST exhibited better antigenicity in silico
  • Gene expression profiles supported that DmGSTM1 and DmGSTM2 are of potential value to be developed as anti-tick vaccine
  • This study offers some insight into finding tick GST as protective antigen; further studies need to be carried out in order to validate the antigenic effect of the DmGSTs
  • This study offers some insight into finding tick GST as protective antigen; further studies need to be carried out in order to validate the antigenic effect of the D. marginatus glutathione S-transferases

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